Differential epigenetic regulation of BDNF and NT-3 genes by trichostatinA and 5-aza-2'-deoxycytidine in Neuro-2a cells.

Differential epigenetic regulation of BDNF and NT-3 genes by trichostatinA and 5-aza-2'-deoxycytidine in Neuro-2a cells.

Biochem Biophys Res Commun. 2010 Feb 24;

Authors: Ishimaru N, Fukuchi M, Hirai A, Chiba Y, Tamura T, Takahashi N, Tabuchi A, Tsuda M, Shiraishi M

To understand epigenetic regulation of neurotrophins in Neuro-2a mouse neuroblastoma cells, we investigated the alteration of CpG methylation of brain-derived neurotrophic factor (BDNF) promoterI and neurotrophin-3 (NT-3) promoterIB and that of histone modification in Neuro-2a cells. Bisulfite genomic sequencing showed that the CpG sites of BDNF promoterI were methylated in non-treated Neuro-2a cells and demethylated following 5-aza-2'-deoxycytidine (5-aza-dC) treatment. In contrast, methylation status of the NT-3 promoterIB did not change by 5-aza-dC treatment in Neuro-2a cells. Furthermore, we demonstrated that BDNF exonI-IX mRNA was induced by trichostatinA (TSA) treatment. However, NT-3 exonIB-II mRNA was not induced by TSA treatment. Chromatin immunoprecipitaion assays showed that the levels of acetylated histones H3 and H4 on BDNF promoterI were increased by TSA. These results demonstrate that DNA methylation and/or histone modification regulate BDNF gene expression, but do not regulate NT-3 gene expression in Neuro-2a cells.

PMID: 20188708 [PubMed - as supplied by publisher]

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Posted 2 Mar 2010 8:32 AM by pubmed: neuroblastoma
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