High expression of large-conductance Ca(2+)-activated K(+) channel in the CD133+ subpopulation of SH-SY5Y neuroblastoma cells.

High expression of large-conductance Ca(2+)-activated K(+) channel in the CD133+ subpopulation of SH-SY5Y neuroblastoma cells.

Biochem Biophys Res Commun. 2010 Apr 30;

Authors: Park JH, Park SJ, Chung MK, Jung KH, Choi MR, Kim Y, Chai YG, Kim SJ, Park KS

Solid tumors contain a population of cancer stem cells (CSCs), and CD133 is widely used as a CSCs marker. We investigated the differences between CD133+ and CD133- cells from the neuroblastoma cell line SH-SY5Y in terms of the expressions of voltage-gated ion channels. A CD133+ enriched (>60 %) population was isolated, and a subsequent whole-cell voltage clamp study showed that these cells predominantly express TEA-sensitive outward K(+) currents (I(K,TEA)) and TTX-sensitive voltage-gated inward Na(+) currents (I(Na)). Cell-attached single channel recordings demonstrated higher density of large-conductance (155 pS) channel in CD133+ cells than in CD133- cells. The TEA-sensitivity and single channel conductance indicated the large-conductance Ca(2+) activated K(+) channels (BK(Ca)). Furthermore, RT-PCR analysis of 22 transcripts of voltage-gated ion channels in SH-SY5Y cells showed the expressions of Cav1.3, Kir2.1, Kv1.4, Kv2.1, Kv4.2, Kv7.1, BK(Ca), and Nav1.7, and those of BK(Ca) and Na1.7 were higher in CD133+ than in CD133- cells. In addition, the increase of cytosolic Ca(2+) concentration ([Ca(2+)](c)) in response to ionomycin (a Ca(2+) ionophore) was higher and more sustained in CD133+ than in CD133- cells. Plausibly membrane hyperpolarization via BK(Ca) might be responsible for the augmented Ca(2+) influx observed in CD133+ cells. The physiological implications of the differential expression of BK(Ca) and Nav1.7 in CSCs require further investigation.

PMID: 20438714 [PubMed - as supplied by publisher]

Read the complete post at http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?tmpl=NoSidebarfile&db=PubMed&cmd=Retrieve&list_uids=20438714&dopt=Abstract

Posted 5 May 2010 3:22 AM by pubmed: neuroblastoma
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